叶黄素对人胃癌与乳腺癌细胞增殖抑制作用与机制

【Abstract】wWw.shuoshilunwen.com Background and Objective Currently, the natural plant chemical study of biological effects has become a hot topic in the area of food and pharmacy research. Researchers try to find biological compounds from plant,do the problem of relieving and resolving various diseases. With the study on lutein’s physical-chemical properties and biological activity going deeply,medical researchers he been attracted more attention on lutein. Numerous epidemiological studies he demonstrated that lutein has extensive biological capabilities in protecting age-related macular degeneration,preventing the development of cancer,cardiovascular disease and enhancing immune functions. But the growth inhibiting effect on gastric cance and breast cancer of lutein has not beening reported. The study try to investigate the growth inhibiting effect of lutein on SGC-7901 cells and MCF-7 cells,the effect of inducing cells apoptosis, then to approach its mechani.Methods The growth inhibiting on SGC-7901 of lutein:after treated with lutein,the proliferation of SGC-7901 cells were detected by MTT assay. Observe SGC-7901 cells by inverted microscope. Cell apoptosis and cell cycle was detected by flow cytometry after the SGC-7901 cells were treated with lutein. Use UV to detect the caspase-3 activity of SGC-7901 after treated with diffrent time. The growth inhibiting on MCF-7 of lutein: the proliferation of SGC-7901 cells were detected by trypan blue exclusion method. Observe MCF-7 cells by inverted microscope. The lutein-induced apoptosis in MCF-7 was detected by DNA-ladder apoptosis assay. After treated with lutein, the DNA damage of MCF-7 was detected by single cell gel electrophoresis assay. Use UV to detect the caspase-3 activity of SGC-7901 after treated with diffrent time.Results The growth inhibiting on SGC-7901 of lutein:lutein resulted in significant inhibition of SGC-7901 cells in a dose-dependent manner. Lutein groups compare with the DMSO control group,their cell density decrease,partly of them cell volume reduce,cytopla and cell nucleus become concentrated. The majority of cells chang their form. Growth over time,more obvious changes in cell toxicity. Flow cytometry assay suggested that lutein can induce SGC-7901 cells apoptosis and change its cell cycle. Lutein can increase ’SGC-7901 cell’s caspase-3 activity,18h reaches the peak,48h after is still higher than corresponding control group cells. The growth inhibiting on MCF-7 of lutein: lutein resulted in significant inhibition of MCF-7 cells in a dose-dependent manner. Cell morphology observed with the same results of SGC-7901. After treated with lutein 48h, the DNA damage of MCF-7 was detected by single cell gel electrophoresis assay,lutein cells show comet-like electrophoretogram. Lutein may result in MCF-7 DNA damage. After treated with lutein 30h,extracted DNA from cells,obvious DNA ladder can be found,this show lutein can induce apoptosis of MCF-7. lutein can increase MCF-7 cell’s caspase-3 activity.Conclusions Lutein can inhibit the proliferation of SGC-7901 cells effectively,cause the change of cell cycle and SGC-7901 cells apoptosis appeared,increase the caspase-3 activity. Lutein can inhibit the proliferation of MCF-7 obviously,damage the DNA of MCF-7 and reduce apoptosis, increase the caspase-3 activity.